How is colorimetry used in aspirin?
How is colorimetry used in aspirin?
The amount of 2-hydroxybenzoic acid in a solution made by hydrolysing aspirin can be determined by adding iron(III) ions and measuring the intensity of the violet-blue solution. From this the amount of aspirin can be calculated. Colorimeter and suitable filter (green/yellow).
What wavelength does aspirin absorb at?
The absorption spectra of aspirin and salicylic acid in phosphate buffer (pH 6.8) are shown in Fig. 1. The absorption peaks were at 233 nm and 296 nm. At the wavelength of 265 nm, aspirin and salicylic acid had a different absorbance, so it affected the determination of dissolution rate.
How do you measure aspirin content?
Aspirin is a weak acid that also undergoes slow hydrolysis; i.e., each aspirin molecule reacts with two hydroxide ions. To overcome this problem, a known excess amount of base is added to the sample solution and an HCl titration is carried out to determine the amount of unreacted base.
What wavelength should be used for the detection of aspirin via spectrophotometry?
530 nm
Sample methods for Spectrophotometric Analysis of Aspirin The absorbance and transmittance of the five solutions were measured at 530 nm. The mass of ASA in commercial pain reliever tablets was determined using spectrophotomeric analysis.
What instrument measures sample color?
A spectrophotometer measures the spectral reflectance, transmittance, or relative irradiance of a color sample. A spectrocolorimeter is a spectrophotometer that can calculate tristimulus values.
How do you identify salicylic acid?
- Salicylic Acid is a beta hydroxy acid that occurs as a natural compound in plants.
- Salicylic acid is an odorless white to light tan solid.
- Salicylic acid is a monohydroxybenzoic acid that is benzoic acid with a hydroxy group at the ortho position.
What is the spectrophotometric analysis of aspirin?
SPECTROPHOTOMETRIC ANALYSIS OF ASPIRIN. Introduction: A colored complex is formed between aspirin and the iron (III) ion. The intensity of the color is directly related to the concentration of aspirin present; therefore, spectrophotometric analysis can be used.
Which wavelength range is associated with UV spectroscopy 0.8 500.0 400 100nm 380 750nm 0.01 10nm?
400 to 100 nm is connected with UV radiates. 0.8 – 500µm is in the IR spectroscopy range, 380 – 750nm is the undeniable spectroscopy range and 0.01 10nm is the X-bar range.
How do you test the purity of aspirin?
Because today’s aspirin is mass produced in tablet form, Spectrophotometers are a reliable and economical way to keep the purity of Aspirin consistent throughout the manufacturing process. The purity and amount of acetylsalicylic acid in aspirin can be measured using a Visual Spectrophotometer.
What is the percent purity of aspirin?
The aspirin was 99.6 % pure.
What is lab in color measurement?
The CIELAB space is three-dimensional, and covers the entire range of human color perception, or gamut. It is based on the opponent color model of human vision, where red and green form an opponent pair, and blue and yellow form an opponent pair.
Is colorimetry quantitative or qualitative?
Colorimetry is a simple, quick, and easy method for the analysis of a sample. It is based on a chemical reaction between the analyte and an appropriate reagent to produce a visible colored product. It is commonly used as qualitative analysis to indicate the presence or absence of an analyte of interest within a sample.
Is there a colorimetric method to detect aspirin?
Since free SA is significantly toxic for humans, the content determination of free SA is absolutely necessary to ensure people’s health. In this work, a facile colorimetric method was developed for the detection of SA in aspirin by utilizing the MIL-53 (Fe) nanozyme.
Why is the impurity of salicylic acid in aspirin important?
The impurity of salicylic acid (SA) in aspirin is a required inspection item for drug quality control. Since free SA is significantly toxic for humans, the content determination of free SA is absolutely necessary to ensure people’s health.
What causes the discoloration of free aspirin?
Besides, the phenolic hydroxyl group in the free SA is readily oxidized and consequently form a series of colored quinone compounds, leading to discoloration of aspirin. There have corresponding safety limits for free SA in aspirin in countries around the world.
How is sa produced in the production of aspirin?
Aspirin, as a widely used drug, has the effects of an antipyretic, analgesic and anti-inflammatory ( Dahl and Kehlet, 1991; Parham and Rahbar, 2009 ). However, SA can be easily produced due to the incomplete acetylation in the production process of aspirin or hydrolysis during the refining process and storage.
