Do primary microglia proliferate in culture?
Do primary microglia proliferate in culture?
Contribution of GM-CSF to microglia proliferation in culture Regardless of passage, microglia cultured in the presence of GM-CSF proliferated as described, reaching 70–80% confluence within 7–12 days, allowing continuous splitting to higher passages.
How many microglia are in a mouse brain?
Typical yield is from 300,000 to 500,000 microglial cells per mouse brain (see Fig. 2).
What are BV2 microglial cells?
BV-2 is a type of microglial cell derived from C57/BL6 murine. The BV2 cells are immortalized by v-raf/v-myc carrying J2 retrovirus. BV2 express nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level.
Are there different types of microglia?
According to their shape, microglial cells have been categorized into three broadly distinct subtypes: compact, longitudinally branched and radially branched (Lawson et al., 1990). These morphologies are closely related to their functional state (Davis et al., 1994).
What are primary microglia?
Primary microglia, in either mono-culture or co-culture with neurons or astrocytes, are a powerful tool for studying mechanisms underlying microglial inflammatory responses and cell type-specific interactions in the central nervous system (CNS).
What are the functions of microglia?
Microglia are resident cells of the brain that regulate brain development, maintenance of neuronal networks, and injury repair.
What are RAW cells?
RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. RAW-Blue™ cells express the secreted alkaline phosphatase (SEAP) reporter gene.
How do you identify microglia?
A combination of CD11b and CD45 labeling can be used to distinguish microglia from macrophages. Resting microglia are CD11bhi, CD45low, whereas macrophages are CD11bhi, CD45hi.
Where does the microglia in a mouse come from?
Mouse Microglia (MMcg) are primary mouse microglia derived from day 1 postnatal CD1 mouse brains. When revived and plated and cultured under recommended conditions, MMcg form adherent culture.
How does microglia respond to LPs and I : C?
Microglia, CNS resident innate immune cells, respond strongly to activation of TLR3 and TLR4, which recognize viral dsRNA poly (I:C) and bacterial endotoxin LPS, respectively. However, few studies have thoroughly and parallelly compared functional phenotypes and downstream mechanisms between LPS- and poly (I:C)-exposed primary microglia.
Where does MMCG come from in the mouse?
Mouse Microglia (MMcg) are primary mouse microglia derived from day 1 postnatal CD1 mouse brains. When revived and plated and cultured under recommended conditions, MMcg form adherent culture. Microglia, originated from the hematopoietic stem cells in bone marrow, are the resident macrophages in the central nervous system (CNS) which is
