What is H9c2 cells?
What is H9c2 cells?
H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.
What does transient transfection mean?
Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell. With the growth and division of cells, foreign genes will be gradually and totally lost.
What is forward and reverse transfection?
The key difference between forward and reverse transfections is the cell density at the time of plating. Cells lose a day of doubling in a typical reverse transfection protocol; therefore, approximately twice the number of cells are recommended compared to a forward transfection.
How do you differentiate H9c2 cells?
H9c2 cells have the ability to differentiate towards a cardiac phenotype when the media serum is reduced in the presence of all-trans-retinoic acid (RA), creating multinucleated cells with low proliferative capacity.
What is C2C12 cell line?
The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977. These cells are capable of rapid proliferation under high serum conditions and differentiation into myotubes under low serum conditions.
What does stably transfected mean?
Stably transfected cells pass the introduced DNA to their progeny, typically because the transfected DNA has been incorporated into the genome, but sometimes via stable inheritance of nongenomic DNA.
What is transient transfection used for?
Transient transfection can be used to investigate the short-term impact of altered gene or protein expression. Transiently transfected genetic material is not integrated into the host genome and therefore the effect on target gene expression is time-limited and is lost during ongoing cell division.
What is meant by transfected?
Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.
Is reverse transfection better?
Advantages and disadvantages. The advantages of reverse transfection (over conventional transfection) are: The addition and attachment of target cells to the DNA-loaded surface can lead to a higher probability of cell-DNA contact, potentially leading to higher transfection efficiency.
How are H9C2 cells used as transfection hosts?
Cells from the H9c2 cell line express ACh receptors and are often used as transfection hosts in the study of many myocardial diseases. Altogen Biosystems produces pre-optimized lipid-based high-efficiency transfection reagent kits for the H9c2 embryonic rat myocardium cell line.
Where was the H9c2 cell line originally isolated from?
The H9c2 cell line was initially isolated from the ventricular part of a BDIX rat heart [ 2 ]. Thirteen days after fecundation, cells were isolated and immortalized.
Are there two differentiation states in H9C2 cells?
In the present study, we performed for the first time a transcriptional analysis of the H9c2 cell line in two differentiation states, i.e. embryonic cells and differentiated cardiac-like cells.
What is the density of a H9C2 cell?
H9c2 cells were plated at a density of 5,000 cells/ml, for non-differentiated conditions (10% FBS) or at 35,000 cells/ml for cell differentiated conditions (1% FBS + RA) in 75 cm 2 tissue culture flasks and cultured for one more day in high serum in order to allow for cell attachment.
