What is Jurkat T cell?

What is Jurkat T cell?

From Wikipedia, the free encyclopedia. Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.

Why are they called Jurkat cells?

Cell Line Description Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called “JM” (JURKAT and JM are derived from the same patient and are sister clones).

Is Jurkat CD4 or CD8?

Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.

Is Jurkat a CD4?

Jurkat is a CD4 T cell line provided by O. Acuto (Institut Pasteur, Paris, France).

Are Jurkat cells CD8 positive?

Jurkat cells are a CD8-negative human T-cell lymphoma and T2 cells are a Tap-1−/− human HLA-A*0201 B/T lymphoma. Melanoma cell lines, mel 1300 and mel 624, are HLA-A2 positive, whereas mel 888 is HLA-A2 negative ( 31).

Are Jurkat cells cytotoxic?

There are cytotoxic CD4 T cells. But if the CD4 T cell line used is the Jurkat T cell, is it possible to measure the cells’ cytotoxicity against their target cells (these Jurkat T cells have been modified to recognize certain tumor surface Ag?)

Are Jurkat cells CD3 positive?

I noticed that although Jurkat cells are T cells, their positive signals for CD3 antibodies were not so high. When stained, the signal does shift to the right, but does not shift so dramatically that when I try to gate, some (30-40%) of the population still remain in the region where unstained cells sit.

Do Jurkat cells express CD8?

All Answers (1) The Jurkat T cell leukemic line E6–1 expresses high levels of mature TCR and low levels of CD4, but no CD8 or MHC class II.

Do Jurkat cells express CD25?

It has been found that in growing Jurkat culture within 24 h, phytohemagglutinin (PHA, 5 μg/ml) or PHA in combination with 12,13-phorbol dibutirate (PDBu, 10(-8)M) increase the number of CD25+ cells to 32.3 ± 3.4% (n = 11) and 44.8 ± 8.6% (n = 6) respectively.

Are Jurkat cells suspension or adherent?

Jurkat Clone E6-1 is a human T lymphoblastoid cell line derived from an acute T cell leukemia. The cells are suspension lymphoblasts.

Which structure will express CD8 surface antigen by immunohistochemistry?

The CD8 co-receptor is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. The CD8 molecule is a marker for cytotoxic T cell population.

How do you activate Jurkat cells?

Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works.

Where does the Jurkat cell line come from?

The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14].

How are Jurkat cells cultured in a centrifuge?

Jurkat cells or TA3Ha cells are cultured at 37°C under 5% CO 2 in cell growth medium until cells reach confluency. The mixture of cells in cell growth medium is transferred to a conical centrifuge tube. The centrifuge tubes with cells are centrifuged at 1600 rpm for 5 min at 4°C.

How are Jurkat cells used in T cell activation?

In T cell signaling [ 15 ], the Jurkat cell line has been used to model and characterize signaling events in T cell activation (TCA), a critical process in effective adaptive immune response (also see [ 14 ]). A PubMed search using the terms ‘Jurkat’ and ‘T cell activation’ yielded over 6000 publications.

How is the recovery period for Jurkat cells?

After the initial analysis, nutrient-starved Jurkat cells are centrifuged and resuspended in RPMI media for a 1 h recovery period. After recovery, the Jurkat cells are stained and analyzed again by Cellometer and flow cytometry.