What is GelGreen used for?
What is GelGreen used for?
GelGreen® is a highly sensitive, non-toxic green fluorescent nucleic acid dye designed for staining DNA in agarose gels.
How does GelGreen work?
When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA. The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide. GelGreen is sold as a solution in either DMSO or water.
How does GelRed work?
How does GelRed Work? Like ethidium bromide, GelRedTM is a nucleic acid dye that intercalates with DNA. And it binds exclusively by intercalation. When UV light is shown, the intercalated GelRed TM will fluoresce producing a bright orange light.
How does SYBR Safe stain work?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
How do you dilute red gel?
Post staining with GelRed™ is simple, requiring no destaining and no special buffer. Simply dilute the concentrated dye in 0.1 M NaCl or water and incubate the gel in the diluted dye solution for 30 minutes.
What is Biotium?
Biotium is a leading life science reagent manufacturer and supplier devoted to providing high-quality and innovative fluorescent tools that fuel scientific discovery. Many of these technologies have been licensed out to leading life science technology companies worldwide.
Is GelRed cheaper than ethidium bromide?
GelRed is more expensive than ethidium bromide but something to consider when doing a cost comparison is some labs may be able to save on hazardous waste disposal costs (as well as the time and hassle of decontaminating electrophoresis buffers) by switching from EtBr to GelRed.
What is the purpose of adding the fluorescent dye GelGreen to the agarose gel?
GelGreen® is a sensitive, non-mutagenic, and environmentally safer green fluorescent DNA gel stain. Use GelGreen® for staining and visualising DNA in agarose gels during gel electrophoresis.
What is GelRed made of?
GelRed is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelRed structurally consists of two ethidium subunits that are bridged by a linear oxygenated spacer. GelRed is a fluorophore, and its optical properties are essentially identical to those of ethidium bromide.
Why was it important that SYBR Safe stain was added to the gel solution?
The use of SYBR Safe DNA Gel Stain with non-UV blue light emitted by the Invitrogen E-Gel Imager System with Blue Light Base allows you to purify DNA with virtually no UV-induced nicking or crosslinking, resulting in dramatically increased cloning efficiencies.
How long does SYBR Safe last?
SYBR Safe DNA gel stain is stable for at least 6 months when stored at room temperature.
Is red gel safe?
Non-Mutagenic and Safer for the Environment A series of safety tests have confirmed that GelRed® is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining.
What kind of transilluminator does GelGreen gel stain use?
GelGreen® is compatible with a 254 nm UV transilluminator, and can be imaged using a SYBR® Green or GelStar® filter. It also can be used with visible blue light excitation imagers (blue LED light box or Dark Reader®).
Why are GelRed and GelGreen not mutagenic?
Thus, we engineered the chemical structures of GelRed® and GelGreen® such that the dyes are incapable of crossing cell membranes. The Ames test confirmed that GelRed® and GelGreen® are non-mutagenic at concentrations well above their working concentrations used for gel staining.
How is GelGreen used in DNA electrophoresis?
GelGreen is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelGreen consists of two acridine orange subunits that are bridged by a linear oxygenated spacer. Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes.
How are GelRed and GelGreen Gel stains disposed of?
Furthermore, environmental safety tests showed that GelRed™ and GelGreen™ are nonhazardous and nontoxic to aquatic life. As a result, GelRed™ and GelGreen™ can be disposed of down the drain or in regular trash. GelRed™ and GelGreen™ are highly sensitive either as precast gel stains or post gel stains.
