What does cathepsin B do?
What does cathepsin B do?
Structurally, cathepsin B is a bilobal protein with cysteine, histidine, and aspartic acid forming active site of the enzyme at the interface between the two lobes [37]. Cathepsin B can function as an endopeptidase, cleaving internal peptide bonds, as well as an exopeptidase (carboxydipeptidase activity) [37, 38].
Where is cathepsin located?
Cathepsins B and L are cysteine proteases that belong to the papain subfamily of cysteine proteases. Located predominantly in endolysosomal vesicles, both cathepsins B and L are constitutively expressed and participate in normal protein turnover (Mohamed and Sloane, 2006).
What does cathepsin B cleave?
Cathepsin B is a cysteine protease that acts as an exopeptidase at pH<5.5 and as an endopeptidase at pH>5.5 [3]. Its cleavage specificity prefers, but is not restricted to, basic amino acids at the P1 position and hydrophobic or arginine residues at the P2 position [24].
What is cathepsin S activity?
Cathepsin S (CTSS) is a protease located in lysosomes or endosomes of professional antigen presenting cells (APC), such as macrophages, dendritic cells and B cells1. Dysregulated CTSS expression and/or activity has been reported to be involved in the pathogenesis of various diseases.
Where does cathepsin B cleave?
Which is the best anti cathepsin B antibody?
Anti-cathepsin B Antibody (H-5) is rated 4.6 out of 5 by 14 . Anti-cathepsin B Antibody (H-5) is recommended for detection of cathepsin B of mouse, rat and human origin by WB, IP, IF and ELISA
How many KdA does pro-cathepsin B have?
Cathepsin B is produced from a larger precursor form, pro-cathepsin B, which runs at approximately 44 kDa on SDS-PAGE, and is proteolytically processed and glycosylated to form a mature two-chain protein containing a heavy chain (running at 27 and 24 kDa) and a light chain (5 kDa).
How is a cathepsin B ( G60 ) antibody produced?
Cathepsin B (G60) Antibody detects endogenous levels of total human cathepsin B protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly60 of human cathepsin B. Antibodies are purified by protein A and peptide affinity chromatography.
What kind of disease is cathepsin B associated with?
Cathepsin B is a lysosomal cysteine protease that has been implicated in the pathogenesis of rheumatoid arthritis, muscular dystrophy and tumor metastasis. Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content.
