What is bodipy staining?

What is bodipy staining?

With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.

Is bodipy polar or nonpolar?

Invitrogen™ BODIPY™ dyes are unusual in that they are relatively nonpolar and the chromophore is electrically neutral (Figure 1.4. 2). These properties tend to minimize dye-induced perturbation of conjugate functional properties.

What is BODIPY used for?

BODIPY dyes have been used extensively in recognition and imaging applications owing for their improved stability toward photobleaching as well as high quantum yield (Φ) and high molar absorption coefficient (ɛ) values.

How do you dilute BODIPY?

Dissolve 1.3 mg BODIPY in 1 ml DMSO and can be stored at −20 °C. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.

Is Nile red toxic?

TOXICITY AND IRRITATION NILE RED: No significant acute toxicological data identified in literature search. All waste must be handled in accordance with local, state and federal regulations.

Is Nile Red Dead?

NileRed on Twitter: “I’m not dead.

Is BODIPY a fluorescent?

Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change.

What can BODIPY 505 / 515 be used for?

With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 505/515 can be used as a stain for netural lipds and as a tracer for oil and other nonpolar lipids. For Research Use Only. Not for use in diagnostic procedures.

What is the peak emission of BODIPY 505 / 515?

When excited with a blue laser (450 to 490 nm), BODIPY 505/515 gives a green peak emission ranging from 515 to 530 nm [25,48,49]. According to Cooper et al., (2010) [20], BODIPY 505/515 can be used to visualize lipid droplets within microalgae cells and the fluorochrome permeates all structures within a cell.

Which is better Nile red or BODIPY 505 / 515?

BODIPY 505/515 has been used more recently as a potential alternative to Nile red. In fact, these dyes do not stain all microalgae successfully, even though methods are constantly improving, as reported in the literature.

Which is the best description of BODIPY dye?

BODIPY or boron dipyrromethene is a class of strong ultraviolet-absorbing molecules demonstrating a relatively sharp emission peak. These dyes are rather insensitive to pH and the polarity of their environment.