What is the fractionation range of Sephadex G-75?
What is the fractionation range of Sephadex G-75?
Exclusion chromatography.
| Gel Type | Fractionation Range | |
|---|---|---|
| Globular Proteins | Dextrans | |
| G-75 | 3000–80,000 | 1000–50,000 |
| G-75 SF | 3000–70,000 | 1000–50,000 |
| G-100 | 4000–150,000 | 1000–100,000 |
What is the MW of Sephadex?
Sephadex gel-filtration chromatography and SDS-PAGE have been used for assessment of molecular weight. The molecular weight of the enzyme and its different forms has been reported to be between 23,500 and 35,000 (Baldwin and Pressey, 1989; Bugbee, 1990; Wijesundera et al., 1984).
What is G in Sephadex g25?
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).
How do you use Sephadex LH-20?
In solvents such as chloroform, Sephadex® LH-20 is less dense than the solvent and the medium will float. Pour the medium into the column and drain until the second adapter can be inserted. Lock the adapter in position at the surface of the medium and direct the flow of chloroform upwards.
How does Sephadex chromatography work?
Gel filtration based on Sephadex enables group separation of biomolecules that are above the exclusion limit of the medium, from contaminants such as salts, dyes, and radioactive labels. Sephadex is prepared by cross-linking dextran with epichlorohydrin.
What does fractionation range mean?
The average or maximum effective pore size defines what is called the fractionation range or exclusion limit of the resin. Molecules smaller than the fractionation range can enter the pores of the resin, while molecules larger than the fractionation range are excluded from entering the pores.
What is Sephadex made of?
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range.
What is LH 20?
Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc.
How do you clean a Sephadex column?
Try autoclaving at 120 degrees for 10 min and then wash with 2 column volumes of 0.2 M NaOH (5 min) and then reequilibrate with the gel with 2-3 column volumes of buffer. Store the used gel in 2-8 degrees in 20% ethanol.
Why is Sephadex used in gel filtration?
What is the difference between the various grades of Sephadex?
Sephadex G-10, G-15, G-25, G-50, G-75, and G-100 are based on dextran of different molecular weight ranges. The lower the G-value, the higher the degree of cross-linking of the dextran polymers, and the lower the molecular weight fractionation range.
How does reverse phase chromatography work?
Reversed-phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase, which has a stronger affinity for hydrophobic or less polar compounds. Reversed-phase chromatography employs a polar (aqueous) mobile phase.
What can Sephadex G-75 be used for?
Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom. Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
What is the CAS number for Sephadex G-100?
Sephadex® G-100 Medium. CAS Number: 9050-94-6 MDL Number: MFCD00132235 Application Sephadex® G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography. Legal Information Sephadex is a registered trademark of GE Healthcare.
What kind of gel filtration medium is Sephadex?
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of…
Where did the name Sephadex gel come from?
Sephadex is a trademark for cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin. The name is derived from separation Pharmacia dextran.
