What is Steiner stain?
What is Steiner stain?
The modified Steiner stain is a non-specific silver stain for identifying bacteria in formalin-fixed, paraffin-embedded tissues. The principle behind its use is that bacteria are first sensitized using uranyl nitrate solution, making them able to precipitate silver from a silver nitrate solution.
What does warthin Starry stain?
Warthin – Starry stain is a stain based on silver nitrate used in histology. This type of stain is commonly called silver staining. Warthin – Starry stain is used for the visualization of spirochetes but also for the detection of Helicobacter pylori and microsperediates.
What is Mucicarmine stain used for?
Mucicarmine stain is intended for the staining of mucin. Mucin is a secretion produced by a variety of epithelial cells and connective tissue cells. In certain intestinal inflammations or carcinomas, an excess of mucin is secreted by the epithelial cells.
What is silver staining used for?
Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.
What does colloidal iron stain?
2) Colloidal Iron Colloidal Iron is used to distinguish acid mucins. It is often used to replace the Alcian Blue stain due to its greater sensitivity for acid mucins with detection of very small quantities.
Does silver stain stain DNA?
This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis (PAGE). Sensitivity rivals radioisotopic methods and DNA in the picogram range can be reliably detected.
Why Formaldehyde is used in silver staining?
The nucleation sites in proteins promote the reduction of silver ions by formaldehyde into microscopic grains of elemental silver, enabling their detection. The silver staining allows increased peptide coverage with higher sensitivity, reduced background, and least mass spectrometry interference.
How does H and E staining work?
H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors.
What tissue does Alcian blue stain?
connective tissue
Alcian Blue is a stain that is used to visualize acidic epithelial and connective tissue mucins. Mucins are a type of carbohydrate and are found in the GI tract and respiratory tract.
When should I use silver stain?
In pathology, silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.
Why is silver staining more sensitive?
Silver staining is the most sensitive colorimetric method for detecting total protein. The development process is essentially the same as for photographic film: silver ions are reduced to metallic silver, resulting in a brown-black color. Example gel stained with SilverXpress Silver Staining Kit.
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