What is p p53?
What is p p53?
The p53 tumour suppressor is a short-lived transcription factor that is stabilized and activated in response to a range of cellular stresses including hyper-proliferation and DNA damage (1,2).
Is p53 an antibody?
p53 Antibody (DO-1) is a high quality monoclonal p53 antibody (also designated tumor suppressor protein p53 antibody or TP53 antibody) suitable for the detection of the p53 protein of mouse, rat and human origin.
How does p53 regulate the cell cycle?
P53 forms a homotetrameric transcription factor that is reported to directly regulate ~500 target genes, thereby controlling a broad range of cellular processes, including cell cycle arrest, cell senescence, DNA repair, metabolic adaptation and cell death.
What is the molecular weight of p53?
p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa.
How does P 53 turn on transcription?
p53 is a transcription factor, a protein that turns genes on. The animation illustrates how p53 recognizes and binds to a promoter, a specific region of DNA that initiates the transcription of the adjacent gene. After binding to the promoter, p53 recruits an RNA polymerase to transcribe the gene into mRNA.
What activates p53 activity?
p53 is activated by a variety of cellular stresses, including DNA damage, hypoxia, and mitogenic oncogenes, but the extent to which each signal engages p53 as a tumour suppressor remains unknown.
What activates p53 gene?
The tumour suppressor protein p53 is stabilised and activated in response to ionising radiation. This is known to depend on the kinase ATM; recent results suggest ATM acts via the downstream kinase Chk2/hCds1, which stabilises p53 at least in part by direct phosphorylation of residue serine 20.
What does p53 do in the cell cycle?
p53, also known as TP53 or tumor protein (EC :2.7. 1.37) is a gene that codes for a protein that regulates the cell cycle and hence functions as a tumor suppression. It is very important for cells in multicellular organisms to suppress cancer.
How does MDM2 tags p53 for degradation?
Early studies suggested that the MDM2 oncoprotein negatively regulates p53 activity through the induction of p53 protein degradation. MDM2 serves as an E3 ubiquitin ligase of p53; it catalyzes polyubiquitination and subsequently induces proteasome degradation to downregulate p53 protein level.
What does p53 do?
The p53 gene is one type of tumor suppressor gene. This gene codes for proteins that function as a safety net, preventing abnormal cells from developing into cancer cells.
What is p53 marker?
p53 A tumor suppressor gene located on the short arm of chromosome 17 that encodes a nucleophosphoprotein that binds DNA and negatively regulates cell division; frequently measured as a marker of malignant diseases.
How does p53 cause apoptosis?
Upon activation, p53 directly regulates the transcription of ~500 genes and indirectly regulates many additional genes and thereby controls diverse cellular processes. P53 induces apoptosis in nontransformed cells mostly by direct transcriptional activation of the pro-apoptotic BH3-only proteins PUMA and (to a lesser extent) NOXA.
How is p53 induces apoptosis in DNA damaged cells?
How p53 induces apoptosis in DNA damaged cells? P53 is a 53kd tumor suppressor protein that prevents cell cycle progression of cells with damaged DNA. Apoptotic pathway is activated upon failure of DNA repair mechanisms to correct DNA damage. In this pathway, p53 on activation induces expression of BAX gene or Apaf-1 gene.
