What is a 384-well plate?
What is a 384-well plate?
The Standard Thermo Scientific 384-well plate is compatible with the majority of 384 block PCR and sequencing instruments. It has a maximum well volume of 40 µL and a working volume of 25 µL. This 384-well plate is compatible with all major 384 block thermal cyclers, and it has a cut corner at the A24 location.
How many cells are in a 384-well plate?
13. 500 cells / µL (15,000 cells/well/30 µL), or 500,000 cells/mL, 7 mL of cells is sufficient for half of a 384- well plate, so roughly 3.5 million cells are required to plate at a density of 15,000 cells/well in half of a 384-well plate.
Are PCR plates sterile?
All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA. The plastics are then sterilized to kill bacteria and inactivate RNases and DNases, but sterilization does not remove dust or DNA contamination.
What is the function of well plate?
The well plate has become a standard tool in analytical research and clinical diagnostic testing laboratories¹. ELISA is very common and is the basis of most modern medical diagnostic testing in humans and animals. The wells of the microplate typically hold between tens of nanoliters to several milliliters of liquid.
What are microtiter plates used for?
Microtiter plates are most frequently used for screenings and cultivation of large sample amounts. In order to perform an immunoassay (e. g. ELISA = Enzyme Linked Immuno Sorbent Assay) the bottoms of microtiter plate wells are coated with antibodies to detect specific substances within samples.
How many cells are in a 96 well plate?
Useful information for various sizes of cell culture dishes and flasks
Catalog No. | Cells at confluency* | |
---|---|---|
Dishes | ||
24-well | 142475 | 0.24 x 106 |
48-well | 150687 | 0.12 x 106 |
96-well | 167008 | 0.04 x 106 |
What is a skirted 96-well plate?
96-Well Full-Skirted Plates have a low-profile, thin well wall design and are compatible with PCR and qPCR applications. They feature an SBS footprint, and the plates are stackable for use in automated systems. They feature an SBS footprint, and the plates are stackable for use in automated systems.
What are PCR machines?
The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.
What are 96 well plates?
96-well microplates are used for antibiotic screens, cell-based assays and screening compounds. The flat bottom shape is ideal for microscopic and optical measurements. Our plates are high quality for consistent and reliable results.
What is a 24 well plate?
Description. Clear solid 24 well plates are manufactured in pure polystyrene with low fluorescence and can be used for animal and plant cell tissue-culture studies. The radius edged inner bottom of the wells improves the efficiency of washing. The plates comply with SBS standards.
What is 96-well plate used for?
Industry standard, 96-well plates are designed for all common instruments and can be used for applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.