What does CD45 antibody stain?

What does CD45 antibody stain?

The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is specific for mouse CD45 and suitable for immunohistochemical staining of Zinc-fixed and formalin fixed paraffin sections as well as acetone-fixed, frozen sections.

How do you use antibody stain?

Staining

1. Aliquot 100 µl of 1-100 X 105 cells (use wash buffer above with 0.1% NaN3 added) in Falcon® #2052 or #2054 tubes on ice.
2. Add 20 µl MAb (1-10 µg/ml final concentration, depending on Mab).
3. Incubate 10 – 30 min on ice.
4. Wash again as above with 2 ml cold azide buffer.
5. Resuspend in 100 µl of Secondary Antibody (eg.

What is CD45 test?

CD45 (Leukocyte Common Antigen), IHC with Interpretation – This immunoassay is a first-line screening test to evaluate neoplasms of unknown origin and to help determine whether a cell is derived from a hematopoietic origin. In benign tissue or cell blocks, CD45 can be used as a marker of inflammatory cells.

What is human CD45?

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes.

What cells are CD45+?

CD45 antigen (leukocyte common antigen), a unique and ubiquitous membrane glycoprotein with a molecular mass of about 200 kDa, is expressed on almost all hematopoietic cells except for mature erythrocytes. However, the biological function of this glycoprotein still remains to be resolved.

Which cells are CD45 positive?

CD45 is a transmembrane protein tyrosine phosphatase located on most haematopoietic cells. It has several isoforms, and haematopoietic cells express one or more of the isoforms—CD45RO, CD45RA and CD45RB. CD45 immunoreactivity is recognised to be highly specific for non–Hodgkin’s lymphomas.

How do you dilute antibodies?

For example; lets say you need a total 3 ml of your primary antibodies, which also means 3000 uL. Now you need them to be diluted as 1:1000, so simply divide 3000 / 1000 which is 3. So take 3 uL from your Primary antibodies stock vial and add into 3000 uL (3 mL) of PBS or any other diluent as per your choice.

Why are antibodies used in staining?

Primary antibodies provide specific recognition of the target antigen. The use of primary antibodies conjugated to fluorophores, without secondary antibodies, is known as direct staining. Direct staining methods save time and allow multiplexing using antibodies raised in the same host species.

How is CD45 activated?

Lck activity is regulated by modulating the conformation of its kinase domain through the phosphorylation of critical regulatory sites (6, 7). CD45 activates Lck by dephosphorylating a tyrosine in its inhibitory C-terminal tail (8–10).

Are B cells CD45+?

Analysis of CD45 by flow cytometry revealed that unstimulated and stimulated B cells expressed homogeneous amounts of total CD45 but that stimulation with IL-5 resulted in a CD44hi, hyaluronate-adherent subpopulation of activated B cells that expressed a markedly altered pattern of expression of exon-specific CD45R or …

Is CD45 a phosphatase?

CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu Rev Immunol. 1994;12:85-116.

How is CD45 antibody used in immunohistochemistry?

CD45 Antibody Staining Protocol for Immunohistochemistry. Description: This antibody reacts with both alloantigens and all isoforms of the CD45 leukocyte common antigen (LCA), also known as Ly-5 or T200, found on all cells of hematopoietic origin, except erythrocytes.

How are white blood cells stained with MACS Antibodies?

The section was stained using CD45 Antibody, anti-human, PE (green) for 10 min at room temperature. Human white blood cells (WBCs) were stained with CD45 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies.

What is the function of CD45 in leukocytes?

CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

What kind of immunohistochemical analysis is ab10558?

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – Anti-CD45 antibody (ab10558) This image is courtesy of an anonymous abreview. Immunohistochemical analysis of formaldehyde fixed human cephalic sections.