Are Cre mice transgenic?
Are Cre mice transgenic?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
What are Cre driver mice?
Cre-driver lines: A special type of transgenic mouse As different neurons have different cellular machinery to activate and express certain genes, Cre-recombinase was targeted to specific neuronal (or other brain cell types) with this in mind.
What is a transgenic mouse line?
Transgenic mice are mouse models that have had their genomes altered for the purpose of studying gene functions. At Charles River, we help hundreds of global customers by delivering study-ready, transgenic mice to meet their research needs.
What does Cre recombinase do?
Cre recombinase is a widely used tool in the field of molecular biology. The enzyme’s unique and specific recombination system is exploited to manipulate genes and chromosomes in a huge range of research, such as gene knock out or knock in studies.
What is the mechanism of action for Cre recombinase in the Cre loxP system?
Concerning the mechanism of Cre-loxP system, a single Cre recombinase recognizes two directly repeated loxP site, then the Cre excises the loxP flanked (floxed) DNA, thus creating two types of DNA with circular, excised and inactivated gene Y (Figure 1A).
What does Cre stand for in Cre recombinase?
recombination
The P1 gene product was named Cre (causes recombination) and the recombination site was named loxP (locus of crossing (x) over, P1).
What is the role of recombinase?
Recombinases are enzymes that catalyse site-specific recombination events within DNA; for example, genetic recombination during meiosis in which recombination serves to generate new combinations of alleles on chromosomes. Recombinases also function in recombinational DNA repair.
What do transgenic mice do?
Transgenic mice can also be used to study gene function or to generate models for human genetic disease, provided that the desired effect can be observed when the transgene is expressed in the presence of the multitude of host genes. In other words, dominant gain-of-function genetic alterations can be studied.
What does Cre recombinase mean?
site specific recombination
Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites).
How to create a transgenic mouse with VE cadherin?
To generate the VE-Cadherin-Cre transgenic mouse, we fused the Cre-recombinase coding region to a regulatory region of the VE-Cadherin promoter (Fig. 1 a).
Which is the best mouse model for cadherin recombination?
VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad).
What is the function of VE-cadherin in mice?
In addition, VE-Cadherin has been implicated in modulation of flow, VEGF signaling, and vascular permeability within a variety of organs in adult mice (Calera et al., 2004; Corada et al., 2001; May et al., 2005; Venkiteswaran et al., 2002 ).
Why is the vecad-cre-ert2 mouse so important?
The VECad-Cre-ERT2 mouse is likely to be a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth. Copyright (c) 2006 Wiley-Liss, Inc.
