What is FLP recombinase used for?
What is FLP recombinase used for?
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism’s DNA under controlled conditions in vivo.
What is Dre recombinase?
Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech.
How does Cre recombinase work?
How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.
Is Cre recombinase reversible?
All recombination events mediated by FLP or Cre are reversible. Because recombination can only occur between target sites of the same sequence in FLEx vectors, the DNA sequence that should be inverted is flanked by two pairs of target sites that differ in their sequence.
What is FRT sequence?
FRT sites are used often (at least in Drosophila) for inducing deletions or “flipping out” of markers in transgenic constructs. When there are two FRTs sequences in tandem, after inducing flippase the DNA sequence that is between these two sites will be deleted.
What is the brainbow?
Brainbow is a genetic cell-labeling technique where hundreds of different hues can be generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent proteins.
What type of recombinase is Cre recombinase?
Type I topoisomerase
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites (1). The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products (2).
How long does it take for Cre recombinase to work?
By lipofection, it would take about one day for the Cre to be expressed and then it should be immediately active.
What is Cre transgenic mice?
A Cre mouse contains a Cre recombinase transgene under the direction of a tissue-specific promoter; a loxP mouse contains two loxP sites that flank a genomic segment of interest, the “floxed” locus. Typically, Cre and loxP mice are produced by using transgenic technology (Nagy 2000).
