What is lipemia interference?
What is lipemia interference?
After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanisms. The most common preanalytical cause of lipemic samples is inadequate time of blood sampling after the meal or parenteral administration of synthetic lipid emulsions.
What chemistry tests are affected by lipemia?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
What causes Lipaemia?
Lipaemic samples are caused by an excess of lipoproteins in the blood, creating a milky/turbid appearance that interferes with multiple biochemical tests and can even cause haemolysis of red blood cells.
How does lipemia sample affect lab results?
How Does Lipemia Impact Laboratory Testing? Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.
Why does lipemia cause hemolysis?
Hemolysis: Hemolysis of erythrocytes is enhanced in the presence of lipemia. This can affect results of individual tests (particularly end point reactions that are not blanked), because hemoglobin will absorb at wavelengths used to detect reactions in the analyzer.
What causes gross lipemia?
The most common preanalytical cause of lipemia is inadequate time of blood sampling after the meal. In the hospital setting a certain proportion of lipemic samples can’t be avoided, since patients are admitted to the emergency services in various times of the day and various intervals since their last meal.
What causes a lipemic specimen?
The most common cause of lipemia is nonfasting, with recent ingestion of lipid-containing meal. More severe lipemia results from a disease condition causing hypertriglyceridemia (eg, diabetes, genetic hyperlipidemia) or recent intravenous infusion of a lipid emulsion.
What causes specimen hemolysis icterus and lipemia?
Hemolysis from ruptured red blood cells (whether in vitro as an artifact or in vivo, as part of an intravascular component to a hemolytic anemia), lipemia (usually due to increased chylomicrons or high concentrations of very low density lipoproteins) and icterus (from hyperbilirubinemia) in a sample can, in some cases.
Why does lipemia in specimen cause an inaccurate hemoglobin value?
Lipemic specimens contain high levels of triglycerides consisting of chylomicrons and very low-density lipoprotein particles, which in turn cause turbidity. This turbidity interferes with light scatter and the absorption of light, resulting in a false increase of hemoglobin determinations.
How does lipemia affect bilirubin?
Lipemia interferes with chemistry tests by the following mechanisms: Light scattering: Results in falsely increased absorbance readings of some analytes, particularly those that are endpoint reactions that are not blanked, e.g. total bilirubin, resulting in high concentrations of bilirubin.
What are the causes of lipemic or Lactescent specimen?
Lipemia (lactescence) is caused by increased triglycerides (usually as chylomicrons and infrequently from high concentrations of very low density lipoproteins).
What is hemolysis lipemia and icterus?
Blood specimens with hemolysis, icterus or lipemia (HIL) are often encountered in routine clinical laboratory practice. An interferent may originate from blood cells or intravenous fluid; for example, the release of potassium from hemolyzed blood cells can increase plasma and serum concentrations of potassium.
How is lipemia an endogenous interference in the laboratory?
After hemolysis, lipemia is the most frequent endogenous interference that can influence results of various laboratory methods by several mechanism … In the clinical laboratory setting, interferences can be a significant source of laboratory errors with potential to cause serious harm for the patient.
Are there written procedures for detection of lipemia?
Written procedures for detection of lipemia, removing lipemia interference and reporting results from lipemic samples should be available to laboratory staff in order to standardize the procedure, reduce errors and increase patient safety.
How does lipemia interfere with the antigen-antibody reaction?
The peak height correlated with the triglyceride concentration suggesting that the interference was present in a dose-dependent manner. Lipemia can also non-specifically interfere in various immunoassays. Lipoproteins can interfere with antigen-antibody reaction by blocking binding sites on antibodies.
What’s the frequency of lipemia in the hospital?
The overall frequency of lipemic samples ranges from 0.5–2.5%, depending on the type of hospital and proportion of inpatient and outpatient samples ( 4 – 6 ). Analysis of preanalytical errors in our laboratory revealed low frequency of lipemic samples (less than 0.5%) ( 5 ). However, in the outpatient unit,…
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