What are standard columns in HPLC?
What are standard columns in HPLC?
Size exclusion HPLC columns are used primarily for the separation of proteins and carbohydrates. Other types of HPLC columns include affinity, ion exclusion, and displacement chromatography columns; a chiral HPLC column can be used to resolve racemic mixtures.
What is the difference between C18 and C8 columns?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.
What is BDS column?
What is BDS Column? BDS column is a type of reverse-phase HPLC column which has blocked –OH groups. This means the hydroxyl groups in this column are deactivated/not free. It is also named as BDS C18 column, and this column is packed with octadecasilane chains. The term BDS stands for Base Deactivated Silica.
What is an LC column?
Liquid chromatography (LC) columns, also known as high performance liquid chromatography (HPLC) columns, are used to separate the individual components of a sample for either purification purposes or for analysis. HPLC columns can be of different sizes and made of either glass or metal.
How is HPLC different from normal liquid chromatography?
HPLC is distinguished from traditional (“low pressure”) liquid chromatography because operational pressures are significantly higher (50–350 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the column.
How are HPLC columns used in pharmaceutical analysis?
These columns are generally not used in the analysis of pharmaceutical compounds. HPLC columns have a different length varying from 30 mm to 250 mm and their particle size or porosity from 3µ to 5µ. These factors affect the analysis of sample, therefore, these are considered important during the HPLC analytical method development.
How are Size Exclusion Columns used in HPLC?
Our size exclusion columns (SEC) are available in tailored pore sizes to cover a broad range of molecular weight. Application Specific HPLC Columns. Designed for key pharmaceutical and environmental applications, these columns utilize novel and unique chemistries to provide superior resolution with ease of use.
Why are silica columns used in liquid chromatography?
Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases. Therefore silica columns can be used to analyze the compounds of different chemical natures.
