What is Akt antibody?
What is Akt antibody?
Akt Antibody detects endogenous levels of total Akt1, Akt2 and Akt3 proteins. The antibody does not cross-react with related kinases.
What is Akt Thr308?
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473.
What is the molecular weight of Akt?
Akt1/2/3 (H-136) is also recommended for detection of Akt1, Akt2 and Akt3 in additional species, including equine, canine, bovine, porcine and avian. Molecular Weight of Akt1: 62 kDa. Molecular Weight of Akt2: 56 kDa. Molecular Weight of Akt3: 62 kDa.
What is P AKT Ser473?
Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
What size is Gapdh?
146 kDa
GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits.
How do I activate my AKT?
Akt phosphorylation triggers its activation. Liu et al. report that during cell cycle progression, under growth factor stimulation and DNA damage, Akt can be phosphorylated by cyclin A2/CDK2, mTORC2 and DNAPK. This new phosphorylation governs pS473 and pT308 to reach Akt activation.
Where does mTORC2 phosphorylate AKT?
mTORC2 phosphorylates AKT in a hydrophobic motif site that is a biomarker of insulin sensitivity. In brown adipocytes, mTORC2 regulates glucose and lipid metabolism, however the mechanism has been unclear because downstream AKT signaling appears unaffected by mTORC2 loss.
What is GAPDH antibody?
This GAPDH antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.
What does GAPDH gene do?
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has long been recognized as an important enzyme for energy metabolism and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Recent studies have shown that GAPDH has multiple functions independent of its role in energy metabolism.
Which is a negative control for phospho T308 Akt antibody?
Immunohistochemical analysis of AKT (phospho T308) expression in paraffin embedded human lung carcinoma tissue, using ab38449 (1/50). Right-hand panel represents a negative control where ab38449 was pre-incubated with the immunizing (blocking) peptide.
Which is the secondary antibody for Akt staining?
Secondary antibody is an anti-rabbit IgG HRP used at a 1/70,000 dilution. Exposure time 60 secs. ab8933 (4µg/ml) staining AKT (phospho T308) in human breast using an automated system (DAKO Autostainer Plus).
What is the dot blot Antibody for pan Akt?
Dot Blot – Anti-pan-AKT (phospho T308) antibody (ab8933, 5 µg/ml). Secondary antibody is an anti-rabbit IgG HRP used at a 1/70,000 dilution. Exposure time 60 secs. ab8933 (4µg/ml) staining AKT (phospho T308) in human breast using an automated system (DAKO Autostainer Plus).
When to use Akt antibody in TBST, RT?
Block in 5% non-fat milk in TBST, RT, 90min. In order to detect a clear signal, treatment is required when using this antibody. Use at an assay dependent concentration. AKT, also known as protein kinase B (PKB), is a serine/threonine protein kinase.
