What is TMB in an ELISA?

What is TMB in an ELISA?

3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate.

What is TMB?

TMB (3,3′,5,5′-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP), a common label conjugated to antibodies for applications such as immunohistochemistry, western blotting, and ELISA. The combination of HRP and hydrogen peroxide causes the oxidation of TMB, resulting in the formation of a blue color.

Is TMB toxic?

Harmful by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

Why does TMB turn blue?

TMB turns blue in presence of H2O2 and Peroxidase. Sometimes metals also act as peroxidase mimic. Just avoid any peroxide and peroxidase in proximity. You should consider preparing a fresh stock.

What is TMB and HRP?

TMB is light sensitive and is therefore packaged in amber bottles to protect the solution from direct sunlight. The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measur- able color change that correlates with analyte level.

What is full form of TMB?

The full form of TMB is Tamilnad Mercantile Bank Limited. With its head office in Thoothukudi, Tamil Nadu, India, TMB is a bank. In 1921, TMB was founded as Nadar Bank, but it was renamed Tamilnad Mercantile Bank in November 1962 to extend its presence far beyond the Nadar community.

Does TMB contain H2O2?

Product Description. TMB solution is a chromogenic reagent for peroxidase, designed for ELISA techniques Manual or Automatic systems. The solution is ready for use and contains: 3, 3′, 5, 5′- tetramethylbenzidine (TMB), hydrogen peroxide (H2O2), stabilizing agents.

Is TMB light sensitive?

Recommended Handling Please note the following precautions when handling neogen’s TMB substrates: Light Exposure -TMB is very light sensitive and direct exposure to sunlight should be avoided. Prolonged exposure of the substrate to light (especially sunlight) should be avoided.

Why does TMB turn yellow?

Chemical basis for TMB oxidation to colored product The diamine/diimine CTC is in rapid chemical equilibrium with a cation radical. Further oxidation of the one electron loss oxidation state CTC form into the two electron-loss oxidation state leads to the formation of the yellow colored diimine form.

Who is the founder of TMB?

Shanmugavel Nadar as Chairman on Nov 04, 1921. The bank was opened by Shri T.V. Balagurusamy Nadar, the then President of the Nadar Mahajana Sangam and the bank threw open its door to the public on Nov 11, 1921 at 9am in Ana Mavanna Building at South Raja Street, Tuticorin.

Is TMB Nationalised bank?

The bank has been expanding its footprint all over India. TMB was rated as the fastest growing Private Sector Bank continuously for the five years from 2010 to 2015. It was also rated as the Best Bank in the years 2013, 2014 and 2015, due to its robust growth….Tamilnad Mercantile Bank Limited.

What is chromogen TMB?

Additional Information. ELISA TMB Stabilized Chromogen is a ready-to-use one-component horseradish peroxidase substrate (HRP) for ELISA plate applications. It can be used in both kinetic assays (optical density monitored over several time points) and in endpoint assays.

What are the features of 1-Step ultra TMB Elisa?

Features of 1-Step Ultra TMB ELISA Substrate: TMB is the most popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The Pierce 1-Step TMB Substrates are one-component substrates that require no preparation before use.

How are TMB conjugates used in ELISA procedures?

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.

Which is Thermo Scientific Pierce ultra TMB Elisa substrate?

Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate (250 mL) detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. 1-Step Ultra TMB is the most sensitive of the chromagenic substrates.

What kind of substrate is used for Elisa?

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm.