What is DR5 auxin?
What is DR5 auxin?
A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element.
What is DR5 GFP?
DR5-GFP expression in vegetative and inflorescence stage pin1 apices. In pin1 , DR5-GFP is highly expressed in differentiated “stem” tissues outside of the meristem, but signal intensity is variable in meristems. Insets show extremes of DR5-GFP expression in pin1 (F).
What is DR5 Arabidopsis?
DR5 is an artificially synthesized and highly active auxin-response element that contains seven tandem direct repeats of 11 bp, including the auxin responsive factor gene-binding site (TGTCTC) (Ulmasov et al., 1997).
What is DR5 promoter?
KEY MESSAGE : Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems. We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants.
What is DR5 :: GUS?
Gene function: synthetic auxin-responsive promoter (containing response elements such as TGTCTC) fused to the reporter gene gus (beta-glucuronidase). Gene effect: MT- DR5::GUS plants present GUS activity in sites where auxin accumulates (root tips, vascular terminations, shoot and leaf primordium).
Is the dr5 reporter system sensitive to auxin response?
DR5 activity was highest in cortex, suggesting high levels of auxin concentration and/or sensitivity in this tissue. Our study shows that the DR5 reporter system is a sensitive and facile system for monitoring auxin responses and distribution at cellular resolution in poplar.
Which is a reporter gene for the dr5 promoter?
The DR5 promoter driving reporter genes like b-glucuronidase (GUS), green fluorescent pro- tein (GFP) or red fluorescent protein (RFP) has been widely utilized in Arabidopsis, maize, soybean, and tomato to study auxin distribution and response at a cellular level (Sabatini et al. 1999; Gallavotti et al. 2008b; Chaabouni et al. 2009).
How does the DRD5 gene work in the brain?
The DRD5 gene provides instructions for making a protein called dopamine receptor D5, which is found in the brain. This protein works together with a chemical messenger (neurotransmitter) called dopamine. Dopamine fits into the D5 receptor like a key in a lock, which triggers chemical reactions within nerve cells.
How is the dr5 reporter system used in Populus?
KEY MESSAGE : Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems. We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants. We found several similarities with Arabidopsis …
