When to use FuGene as a transfection reagent?

When to use FuGene as a transfection reagent?

FuGENE® HD Transfection Reagent can be used in transfection. protocols in the presence of serum (up to 100%), allowing transfection of cell types that require continuous exposure to. serum, such as primary cell cultures.

How big is a 1mL FuGene HD reagent?

The 1ml size contains sufficient reagent to transfect 333µg of DNA. This is equivalent to over 3,300 wells in. 96-well plates at a 3:1 FuGENE® HD Transfection Reagent:DNA ratio (0.3µl reagent:100ng DNA per well). The actual number of transfections will vary with reagent:DNA ratio, transfection volume and cell type.

Why is it important to use FuGene HD?

With a protocol that is straight-forward and quick, FuGENE® HD allows researchers and scientists to free up valuable lab time. Avoid time-consuming optimization procedures by relying on high transfection efficiency proven in more than 1000 cell types, including many “hard-to-transfect” cells.

What is the ratio of FuGene HD to DNA?

For successful transfection of DNA into cultured cells, the ratio of FuGENE® HD Transfection Reagent:DNA must be. optimized. Ratios of 1.5:1 to 4:1 FuGENE® HD Transfection Reagent:DNA work well with many cell lines, but ratios. outside of this range (4.5:1 to 6:1) may be optimal for other cell types or applications.

How are liposomal reagents used for transfection of cells?

Delivery of nucleic acids into cells using liposomal or multicomponent transfection reagents is a well-established method for transfection of mammalian cell lines. This approach has been widely used (for example, for the overexpression of proteins with expression plasmids or for specific gene knockdown experiments using siRNA).

Which is the best transfection reagent for your lab?

We offer numerous convenient solutions to meet your lab’s needs. FuGENE® HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al. [2009] Biotechniques 47, 617–24).