What is an anti-HA antibody?
What is an anti-HA antibody?
Anti-HA is produced in rabbit using a synthetic peptide corresponding to amino acid residues of the human Influenza hemagglutinin (HA), conjugated to KLH. Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells.
How many kDa is HA tag?
1.1 kDa
The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3).
How long is an HA tag A?
The hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for the infectivity of the human virus.
How many ha tags are there?
Yes, we almost always use only one HA tag and visualize it by IF just fine. We use Abcam9110 anti-HA antibody.
What is HA tag used for?
The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
How do you purify HA-tagged protein?
How do you purify HA-tagged proteins? HA-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The HA-tagged protein binds to the HA-tag specific monoclonal antibody conjugated on an agarose gel.
Can Ha tags be internal?
THE HA tag antibody can detect HA tags in internal, C-terminal, or N-terminal recombinant proteins. It is derived from HA molecule corresponding to amino acids 98-106.
What is HA sequence?
From Wikipedia, the free encyclopedia. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human influenza virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
What is an HA tag used for?
