Which is the best description of multiplex PCR?

Which is the best description of multiplex PCR?

Multiplex PCR: An overview of multiplex PCR assay, primer design for multiplexing, primer design software for multiplex PCR. Multiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment.

Are there any Bio-Rad reagents for multiplex PCR?

Browse a selection of Bio-Rad real-time PCR supermixes optimized for multiplex PCR. Explore Bio-Rad qPCR and RT-qPCR reagents for multiplex PCR and discover the key to optimizing your multiplex PCR assays.

How does QIAGEN multiplex PCR master mix work?

The QIAGEN Multiplex PCR Master Mix includes HotStarTaq DNA Polymerase and a unique PCR buffer containing the novel synthetic Factor MP. Together with optimized salt concentrations, this additive stabilizes specifically bound primers and enables efficient extension of all primers in the reaction without the need for optimization.

What should be the melting temperature for multiplex PCR?

Multiplex PCR assays involve designing of large number of primers, hence it is required that the designed primer should be of appropriate length. Usually, primers of short length, in the range of 18-22 bases are used. 2. Melting Temperature. Primers with similar Tm, preferably between 55°C-60°C are used.

What happens if you take Rituxan and get PML?

Progressive Multifocal Leukoencephalopathy (PML): PML is a rare, serious brain infection caused by a virus that can happen in people who receive RITUXAN. People with weakened immune systems can get PML. PML can result in death or severe disability. There is no known treatment, prevention, or cure for PML.

How is rituximab used to treat lymphoma?

Rituximab (Mabthera, Rituxan) is a chimeric anti-CD20 IgG1 monoclonal antibody (mAb) consisting of human γ1 and κ constant regions linked to murine variable domains (6) . Over the past few years, rituximab has considerably modified the therapeutic strategy for B lymphoproliferative malignancies, particularly non-Hodgkin’s lymphomas (NHLs).

Which is the master mix for TaqMan multiplex PCR?

The TaqMan® Multiplex Master Mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four targets in a single reaction. It is supplied in a convenient 2X concentration premix to perform real-time PCR using TaqMan® probes. The master mix contains: AmpliTaq® DNA Polymerase, UP (Ultra Pure)