What is heating ramp rate?
What is heating ramp rate? A thermal ramp rate was defined as a change in temperature over an interval of time, ramp rate = AT/At. A 1.5 °C/sec or less heating and cooling ramp rate was suggested by the design agency. What is the important of thermal cycling in PCR testing? The Thermal Cycling Process The actual PCR process is conducted via thermal cycling, a process of heating and cooling that creates the conditions necessary for DNA replication. This final increase of temperature allows the Taq polymerase enzyme to actually replicate the template DNA. How is a thermocycler used? Thermocyclers,...