Is glutaraldehyde used for chemical fixation?
Is glutaraldehyde used for chemical fixation? Besides its application as disinfectant and medication, glutaraldehyde is used in biomedical research to fix cells. The principle behind the fixation is the binding of glutaraldehyde to nucleophiles of which the amino groups are the most abundant but binding to, e.g., sulfhydryl groups also occurs (Griffiths, 1993). How do you prepare glutaraldehyde fixation? To prepare 100 mL of glutaraldehyde/paraformaldehyde:Add 2 g paraformaldehyde to approx 35 mL distilled water + 0.5 mL of approx. Heat the parafomaldehyde solution in a fume cupboard to 60°C when the paraformaldehyde dissolves (it is unnecessary to use a thermometer).Cool...