What is the purpose of the confocal pinhole?
What is the purpose of the confocal pinhole? What is the purpose of the pinhole in a confocal microscope? Confocal microscopes allow to optically separate slices of the sample's response and record theses slices as images. Optimally, the slices cover just the depth of focus generated in the microscope. What advantages does the pinhole add to confocal microscopy? By having a confocal pinhole, the microscope is really efficient at rejecting out of focus light. The practical effect of this is that your image comes from a thin section of your sample, this provides you with a small depth of field....