What is MeDIP sequencing?
What is MeDIP sequencing? Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) or DNA immunoprecipitation sequencing (DIP-Seq) is commonly used to study 5mC or 5hmC modification. Anti-5mC antibodies are incubated with fragmented genomic DNA and precipitated, followed by DNA purification and sequencing. How does MeDIP seq work? Methylated DNA immunoprecipitation (MeDIP) Genomic DNA is extracted (DNA extraction) from the cells and purified. The purified DNA is then subjected to sonication to shear it into random fragments. This sonication process is quick, simple, and avoids restriction enzyme biases. What is hMeDIP? The hMeDIP Kit is designed to immunoprecipitate and enrich for DNA fragments containing...