What happens at a restriction site?
What happens at a restriction site? They recognize and bind to specific sequences of DNA, called restriction sites. When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule. Typically, the cut is at or near the restriction site and occurs in a tidy, predictable pattern. How does restriction digest work? Restriction digestion is accomplished by incubation of the target DNA molecule with restriction enzymes - enzymes that recognize and bind specific DNA sequences and cleave at specific nucleotides either within the recognition sequence or outside of the recognition sequence. How can you...